Kit iyi inoshandiswa in vitro qualitative nucleic acid yekuona Monkeypox Virus uye nechepakati/kumadokero kweAfrica clade muHuman serum, lesion exudate samples uye scab specimens.MaPrimer sets uye maprobe akanyorwa neFAM akagadzirirwa kunyatsoonekwa yeMonkeypox Virus, ROX yakanyorwa probe yepakati peAfrica clade, CY5 yakanyorwa probe yeWest Africa clade.Yemunhu RNase P gene yakatorwa panguva imwe chete neyeyedzo sampuli inopa yemukati kutonga kuti isimbise nucleic yekubvisa maitiro uye reagent kutendeseka.Probe yakanangana nemunhu RNase P gene yakanyorwa neVIC.
| Zvikamu | 48 Miedzo/kiti | 96 Miedzo/kiti |
| PCR reaction buffer | 672 μL × 1 chubhu | 672 μL × 2 chubhu |
| PCR enzyme musanganiswa | 50 μL × 1 chubhu | 100 μL × 1 chubhu |
| Positive control | 100 μL × 1 chubhu | 200 μL × 1 chubhu |
| Negative control | 100 μL × 1 chubhu | 200 μL × 1 chubhu |
1. Sensitivity: 200 makopi / mL.
2. Kunyatsojeka: Hapana kusangana neEnterovirus(EV), Measles virus(MV), Rubella virus(RV), Varicella-zoster virus(VZV), Dengue virus(DenV), Human Parvovirus B19(HPVB19), Epstein-barr virus (EBv), hutachiona hwemunhu 6 (HHV-6)
3. Kururamisa: CV ≤ 5%.
Real-Time PCR System:Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX96 TouchTM/iQTM ，Hongshi SLAN -96S/96P,AGS8830, AGS4800